Regulatory

Part:BBa_K2605005

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


FRT2

A FLP recombinase recognition target (FRT) site. This part contains a SapI restriction site 5' of the FRT site and AflII, PvuI, FseI, MfeI, and NsiI restriction sites 3' of the FRT site. That is because this FRT site was designed and constructed as one half of our Multiple-Cloning Site flanked by FRT sites Part BBa_K2605002


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 13


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Categories
Parameters
None